The biacore t200, biacore x100, biacore s200 and biacore 8k biosensors have a predefined injection command to perform sck assay, but is currently unavailable for the biacore 4000 biosensor. Biacore t200 instrument handbook describes the instrumentation in the biacore t200 system, with instructions for operation, maintenance and troubleshooting. Biacore t100 gxp handbook describes how the biacore t100 system is used. Biacore t200 instrument handbook 28976863 edition ac. Biacore 3000 is the highest performance research system available for labelfree studies of biomolecular binding and an ideal system for all research laboratories, providing high quality data on the kinetic and affinity parameters for a given biomolecular interaction. Biaevaluation software handbook br100229 edition ah 5 introduction 1 1 introduction biaevaluation is a standalone software package for presentation and evaluation of sensorgram data from biacore analyses. Biacore spr technology cytiva formerly ge healthcare life. The system delivers high quality kinetic, affinity, concentration, specificity, selectivity, and thermodynamic interaction. Reichert sr7500dc biacore x100 biacore t200 number of channels 22 4. Labelfree interaction analysis ge healthcare fig 1. A sensor surface handbook, from biacore, is available for users requiring more details and information regarding other surface generation teqniques, e. How do you access the data of the sensogram curves. The biacore t200 is an instrument for surface plasmon. Twoday course covering interpretation and evaluation of kinetic and affinity data for biacore t200, biacore t100, and biacore x100 users.
Kinetic and affinity analysis with system specific evaluation software. Biacore t200 gxp handbook describes how the biacore t200 system is. Biacore t200 immunogenicity handbook describes the use of. Extending the throughput of biacore 4000 biosensor to accelerate kinetic analysis of antibodyantigen interaction. Biacore spr technology cytiva formerly ge healthcare. Biacore 3000 complete system with biacore 3000 control software 3. The analyte is the interacting partner that is injected in solution over the sensor surface. Using the surface plasmon resonance spr phenomenon. Extending the throughput of biacore 4000 biosensor to. The new t200 control software is newly designed and not compatible with biamethodwriter. Software wizard templates to help speed up assay optimization and routine analyses. Functions for gxp support are described in a separate biacore t200 gxp handbook. Legal information regarding software licensing and details for acquiring additional licenses. The system efficiently delivers highquality information, whether it is for the characterization of single interaction partners or focused screening of hundreds of samples.
Biacore experiment performed using aminecoupling chemistry. Biacore t200 biacore t200 fig 1 is a versatile, labelfree system for detailed studies of biomolecular interactions, from early research to drug discovery and development, and on to qc. The biamethodwriter is a program to write the biacore automation methods in a more interactive way. In this unit, examples and recommendations for studying protein interactions with a variety of molecules are provided. Measurement of concentration isdiscussed in a separate handbook biacore concentration analysis handbook. Compare the reichert sr7500 dual channel system to the. Biacore t200 software handbook describes how to use the software for creating and editing methods, running experiments and evaluating results. Evaluation of calibrationfree concentration analysis provided by biacore systems. Biacore t200 software handbook 28976878 edition aa. A wedge of polarized light, covering a range of incident angles, is directed toward the glass face of the sensor surface.
Evaluation of calibrationfree concentration analysis. Cross new frontiers in labelfree interaction analysis with biacore. Ge healthcare labelfree interaction analysis biacore 8k. Oct 12, 2015 this video show you how to startup and prepare a biacore t200 system for use. Biacore assay handbook ge healthcare life sciences pages 1. Online help in the biacore t100 control software and evaluation software provides detailed assistance on using the software. Biacore t200 gxp handbook describes functionality added with the optional gxp package, together with some recommendations for using the system in a regulated environment. The options that the ge biacore software provides are not useful, export curves just export a bitmap with a very bad quality by the way and. Twoday course covering interpretation and evaluation of kinetic and affinity data for users with biaevaluation software. Additional features biacore t100 software version 2. Softwareassociated documents such as software notes are available for download via the relevant software folders below. The department of physiology and biophysics school of. Biacore t200, biacore t200 control software version 2.
Biacore t200 instrument handbook, the instruction manual for operating the biacore t200 instrument biacore t200 software handbook, the instruction manual for biacore t200 control and evaluation software what to bring for your first experiment running buffer ligand molecule 2. I have successfully immobilized around 0 ru of a biotinylated protein 24 kda onto a biacore t200 sa chip however, when i flow peptide 800 da over the surface at. This program uses popup menus and dialog boxes to fill in the different parts. Overview of biacore systems and their applications. Use this handbook together with the specific user documentation for your biacore system, which will describe how to implement the assay principles in the instrument and system software. Biacore spr biosensors, which measure realtime analysis of biospecific interactions without the use of labeled molecules, can be used for a wide variety of protein interaction assays. About file types supported by biacore evaluation software. Basic introduction to biacore x100 to help you set up, develop and run your assays. Software associated documents such as software notes are available for download via the relevant software folders below. Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Biacore maintenance kits are convenient kits for proper instrument maintenance. Its technology is often used to measure not only binding affinities, but kinetic rate constants and thermodynamics as well. Biacore 3000 primed to perfection for charaterization. Similar principles can be applied in evaluating pairwise mapping results from other biacore.
Handbooks please note, only handbooks related to systems covered by your registered product keys will be displayed here. Biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Ge healthcare labelfree interaction analysis biacore t200. I have successfully immobilized around 0 ru of a biotinylated protein 24 kda onto a biacore t200 sa chip however, when i flow peptide 800 da over the surface at a concentration of 20 um i. Overview of biacore systems and their applications jason. Any functionality that is added in optional addon modules is described in separate documentation. This handbook describes biacore t100 control software and evaluation software, version 2. Latest software updates and software notes software upgrade packages and additional licences software wizard templates to help speed up assay optimization and routine analyses handbooks covering system operationmaintenance and qflex kits system brochures and application notes. Biacore t200 getting started guide integrated structural biology. The handbook, together with the associated reagent kit, will take you through.
Biacore assay handbook ge healthcare life sciences. Biacore 8k software exhibits a flat interface that provides overview and intuitive, rapid operation. Reflected light is detected within a biacore system. A cfca experiment typically involves injection of a sample at two widely different flow rates. Biacore t100 nanobiophysics usc dana and david dornsife. For company and institution core facilities, proteomics groups, cancer research facilities. This provided us the rationale to develop the sck assay on the biacore 4000. When you are interested in ligand fishing, molecular recognition, biams or other advanced applications. In biacore systems, the surface plasmon resonance spr phenomenon occurs when polarized light, under conditions of total internal reflection, strikes an electrically conducting gold layer at the interface between media of different refractive index. Biacore t200 instrument with reagent rack 2 and sample and reagent. The system provides a comprehensive suite of software tools for setting up, executing, and evaluating biomolecular interaction experiments. Ge biacore t200 getting started guide ligand analyte. With a single click, the compact designed equipment offers realtime run control and.
Ligand, analyte and capturing molecule in relation to the sensor surface. First step for biacore insight evaluation software giant step for biacore systems ever needed to prepare and present spr data for the team at very short notice. This video guides you through the evaluation workflow of a calibrationfree concentration assay cfca in the biacore t200 evaluation software. The software offers a range of powerful tools for confident and reliable interaction analysis suitable for users of all levels of experience. Biacore t100 software handbook describes how to use the software for creating and editing methods, running experiments and evaluating results. An electric field intensity, known as an evanescent wave, is generated when the light strikes the glass. Biacore t200 gxp handbook describes how the biacore t200 system is used with the optional gxp package, for compliance with current gxp regulations. Biacore t100 software version 2 university of oxford.
The sensor chip is then docked into the instrument through a software command. This video show you how to startup and prepare a biacore t200 system for use. Christina burtsoff asp marketing manager ge healthcare. This edition of the biacore x100 handbook applies to systems running software version 2. Biacore t200 software handbook describes how to use the software for creating and editing. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software.
We spend countless hours researching various file formats and software that can open, convert, create or otherwise work with those files. Biacore t100 instrument handbook describes the instrumentation in the biacore t100 system, with instructions for operation, maintenance and troubleshooting. In biacore instruments this surface is one side of a microfluidic flow cell 20 60 nl. Nov 22, 2016 this video guides you through the evaluation workflow of a calibrationfree concentration assay cfca in the biacore t200 evaluation software. Biacore is a life science products company, based in sweden. This eight needle highsensitivity surface plasmon resonance spr system rapidly provides kinetics and. The biacore 2000 3000 is a fully automated spr biosensor system ideal for assay development and fast and accurate routine applications like kinetic analyses of proteinprotein and antibodyantigen interactions as well as accurate concentration measurements. To allow the detection of an interaction one molecule termed by biacore as the ligand is immobilized onto the sensor surface. The binding of the histidines relies on a ntachelated nickel atom. Biacore t200 processing unit 28975001 biacore t200 gxp package 28977954 biacore t200 upgrade kit 28977954 biacore t200 gxp upgrade kit 28977964 biacore t200 software v3.
Biacore t100 gxp package will ensure the highest level of gxp support and 21 cfr part 11 compliance. Biacore t200 software handbook, the instruction manual for biacore t200 control and evaluation software what to bring for your first experiment running buffer ligand molecule 2 50 gml in immobilization buffer, higher concentration if scouting conditions for immobilization. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. Labelfree interaction analysis ge healthcare biacore 8k biacore 8k efficiently delivers binding data with the quality you expect, meeting your toughest challenges in small molecule and biotherapeutic screening and characterization. Biacore t200 evaluation software enables kinetic and. To access this section, you need to be logged in and have a valid product key registered in your account details. Apr 04, 2018 first step for biacore insight evaluation software giant step for biacore systems. Biacore t200 getting started 28984098 edition ab 5 biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200. First step for biacore insight evaluation software. Support for use in regu1ated gxp1 environments is provided in an optiona1 package that adds appropriate. Kinetic and affinity analysis with biaevaluation software. Evaluation is divided into five distinct steps, summarized below. Instrumentspecific and general methodology handbooks can be accessed from the handbooks download page.
459 562 1314 76 1462 1019 1225 1425 1375 224 1371 237 13 1028 281 586 166 271 511 341 607 384 1088 565 661 1068 798 1412 41 1295 1341 392 454 556 1326 670 741 1035 239 129 1432 864 558 1225 1126 819 942